Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells

Abstract : Grape marc extract (GME) showed elicitor activity on suspension-cultured cells of tobacco. The BY-2 cells reacted to GME (0.25% and 0.125%) with a long-sustained pH rise in their growth medium. Using EGTA or LaCl3, we showed that extracellular alkalinization depended on Ca2+ mobilization. The tobacco BY-2 cells challenged with GME promoted cell death and the upregulation of defence-related genes such as PR3, PAL and CCoAOMT. Cell death rate was quantified using an experimental calibrated Evans Blue assay. The GME-induced cell death was dose-dependent and occurred in 24 h. Longer exposure increased the extent of tobacco cell death. To investigate a potential hypersensitive reaction, we tested the effect of various inhibitors of protein synthesis (cycloheximide) and proteases (aprotinin, pepstatin and E-64) on GME-induced cell death. All these chemicals reduced GME-induced cell death rate in 30 min. Overall, our findings indicate that GME elicits early perception events, defence reactions and cell death requiring protein synthesis and proteases.
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Plant Physiology and Biochemistry, Elsevier, 2014, 77, pp.84-89. 〈10.1016/j.plaphy.2014.01.021〉
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Razik Benouaret, Eric Goujon, Pascale Goupil. Grape marc extract causes early perception events, defence reactions and hypersensitive response in cultured tobacco cells. Plant Physiology and Biochemistry, Elsevier, 2014, 77, pp.84-89. 〈10.1016/j.plaphy.2014.01.021〉. 〈hal-00981190〉

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