Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity

Abstract : Gravity is a permanent environmental signal guiding plant growth and development. Gravity detection in plants starts with the displacement of starch-filled plastids called statoliths, ultimately leading to auxin redistribution and organ curvature. While the involvement in gravity perception of several actors such as calcium is known, the early mechanisms of gravity perception remain enigmatic. In particular, the nature of the signal detected by the statocytes is a matter of debate. Microgravity is a unique environmental condition offering the opportunity to decipher this topic. In this study, roots of Brassica napus were grown aboard the International Space Station (ISS) either in microgravity or in a centrifuge simulating Earth gravity. The impact of short simulated gravity onset and removal was measured on statoliths positioning and intracellular free calcium was assessed using pyroantimonate precipitates as calcium markers. Results highlight that very low statoliths displacements under 1 µm are associated with an increase of the intracellular free calcium within 10 minutes. This release was triggered by simulated gravity onset but also by its removal, sustaining that statoliths motion and repositioning may be the variables sensed by the statocytes.
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Pré-publication, Document de travail
2017
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https://hal-clermont-univ.archives-ouvertes.fr/hal-01609781
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Soumis le : mercredi 4 octobre 2017 - 06:09:28
Dernière modification le : mercredi 21 mars 2018 - 18:57:40

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  • HAL Id : hal-01609781, version 1

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François Bizet, Veronica Pereda-Loth, Hugo Chauvet, Joëlle Gérard, Brigitte Eche, et al.. Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity. 2017. 〈hal-01609781〉

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