Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity

Abstract : Gravity is a permanent environmental signal guiding plant growth and development. Gravity sensing in plants starts with the displacement of starch-filled plastids called statoliths, ultimately leading to auxin redistribution and organ curvature. While the involvement in gravity sensing of several actors such as calcium is known, the effect of statolith displacement on calcium changes remains enigmatic. Microgravity is a unique environmental condition offering the opportunity to decipher this link. In this study, roots of Brassica napus were grown aboard the International Space Station (ISS) either in microgravity or in a centrifuge simulating Earth gravity. The impact of short simulated gravity onset and removal was measured on statolith positioning and intracellular free calcium was assessed using pyroantimonate precipitates as cytosolic calcium markers. Our findings show that a ten-minute onset or removal of gravity induces very low statolith displacement, but which is, nevertheless, associated with an increase of the number of pyroantimonate precipitates. These results highlight that a change in the cytosolic calcium distribution is triggered in absence of a significant statolith displacement.
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Article dans une revue
Scientific Reports, Nature Publishing Group, In press
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https://hal-clermont-univ.archives-ouvertes.fr/hal-01609781
Contributeur : Valerie Legue <>
Soumis le : lundi 16 juillet 2018 - 18:52:04
Dernière modification le : jeudi 6 décembre 2018 - 01:27:53

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  • HAL Id : hal-01609781, version 2

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François Bizet, Veronica Pereda-Loth, Hugo Chauvet, Joëlle Gérard, Brigitte Eche, et al.. Both gravistimulation onset and removal trigger an increase of cytoplasmic free calcium in statocytes of roots grown in microgravity. Scientific Reports, Nature Publishing Group, In press. 〈hal-01609781v2〉

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