Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2018

Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli

Martin Herrmann
  • Fonction : Auteur
Alexander Lutsyk
  • Fonction : Auteur

Résumé

A novel mechanism is revealed by which clinical isolates of adherent-invasive Escherichia coli (AIEC) penetrate into the epithelial cell layer, replicate, and establish biofilms in Crohn's disease. AIEC uses the FimH fimbrial adhesin to bind to oligomannose glycans on the surface of host cells. Oligomannose glycans exposed on early apoptotic cells are the preferred binding targets of AIEC, so apoptotic cells serve as potential entry points for bacteria into the epithelial cell layer. Thereafter, the bacteria propagate laterally in the epithelial intercellular spaces. We demonstrate oligomannosylation at two distinct sites of a glycoprotein receptor for AIEC, carcinoembryonic antigen related cell adhesion molecule 6 (CEACAM6 or CD66c), on human intestinal epithelia. After bacterial binding, FimH interacts with CEACAM6, which then clusters. The presence of the highest-affinity epitope for FimH, oligomannose-5, on CEACAM6 is demonstrated using LC-MS/MS. As mannose-dependent infections are abundant, this mechanism might also be used by other adherent-invasive pathogens.
Fichier principal
Vignette du fichier
fmicb-09-00742.pdf (6.75 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01796957 , version 1 (15-11-2018)

Licence

Paternité

Identifiants

Citer

Tetiana Dumych, Nao Yamakawa, Adeline Sivignon, Estelle Garénaux, Stefania Robakiewicz, et al.. Oligomannose-Rich Membranes of Dying Intestinal Epithelial Cells Promote Host Colonization by Adherent-Invasive E. coli. Frontiers in Microbiology, 2018, 9, ⟨10.3389/fmicb.2018.00742⟩. ⟨hal-01796957⟩
217 Consultations
204 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More