Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Journal of Experimental Medicine Année : 2009

Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM

Résumé

Abnormal expression of CEACAM6 is observed at the apical surface of the ileal epithelium in Crohn’s disease (CD) patients, and CD ileal lesions are colonized by pathogenic adherentinvasive Escherichia coli (AIEC). We investigated the ability of AIEC reference strain LF82 to colonize the intestinal mucosa and to induce inflammation in CEABAC10 transgenic mice expressing human CEACAMs. AIEC LF82 virulent bacteria, but not nonpathogenic E. coli K-12, were able to persist in the gut of CEABAC10 transgenic mice and to induce severe colitis with reduced survival rate, marked weight loss, increased rectal bleeding, presence of erosive lesions, mucosal inflammation, and increased proinflammatory cytokine expression. The colitis depended on type 1 pili expression by AIEC bacteria and on intestinal CEACAM expression because no sign of colitis was observed in transgenic mice infected with type 1 pili–negative LF82-fimH isogenic mutant or in wild-type mice infected with AIEC LF82 bacteria. These findings strongly support the hypothesis that in CD patients having an abnormal intestinal expression of CEACAM6, AIEC bacteria via type 1 pili expression can colonize the intestinal mucosa and induce gut inflammation. Thus, targeting AIEC adhesion to gut mucosa represents a new strategy for clinicians to prevent and/or to treat ileal CD.
Fichier principal
Vignette du fichier
J Exp Med-2009-Carvalho-2179-89.pdf (2.34 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-01931313 , version 1 (22-11-2018)

Identifiants

Citer

Frédéric A Carvalho, Nicolas Barnich, Adeline Sivignon, Claude Darcha, Carlos H F Chan, et al.. Crohn's disease adherent-invasive Escherichia coli colonize and induce strong gut inflammation in transgenic mice expressing human CEACAM. Journal of Experimental Medicine, 2009, 206 (10), pp.2179 - 2189. ⟨10.1084/jem.20090741⟩. ⟨hal-01931313⟩
98 Consultations
136 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More