Cigarette smoke condensate affects the retinoid pathway in human amnion

Abstract : Introduction: The preterm premature rupture of membranes (PPROM) is a frequent pathology responsible of more than 30% of preterm births. Tobacco smoking is one of the most frequently described risk factors identified and contributes to the pre term weakening of fetal membranes. As previously demonstrated, all-trans retinoic acid (atRA) regulates several genes involved in the extracellular matrix dynamics, an essential actor in fetal membrane ruptures. We hypothesized that cigarette smoke may affect this pathway in human amnion. Methods: Amnion was obtained from full-term fetal membranes collected from non-smoking women after cesarean births and used either as explants or for the isolation of derived epithelial cells. The pro-healing and transcriptomic effects of atRA were studied by a scratch assay experiment and quantitative RT-PCR, respectively, after treatment with dimethyl sulfoxyde (DMSO), atRA, DMSO þ cigarette smoke condensate (CSC), or atRA þ CSC. Results: Our results show a strong alteration of the retinoid pathway after CSC treatment on amnion-derived epithelial cells and explants. We first demonstrated that CSC inhibits the activity of the RARE reporter gene in amnion-derived epithelial cells. Then, atRA's effects on both the transcription of its target genes and wound healing were demonstrated to be inhibited or at least decreased by the CSC in human amnion epithelial cells. Discussion: Here, we demonstrated that CSC altered the retinoid signal, already known to have roles in fetal membrane physiopathology. These results highlight a potential negative action of maternal smoking on the retinoid pathway in human amnion and more generally on pregnancy.
Type de document :
Article dans une revue
Placenta, Elsevier, 2017, 58, pp.98 - 104. 〈10.1016/j.placenta.2017.08.076〉
Liste complète des métadonnées

Littérature citée [50 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-01614000
Contributeur : Vincent Sapin <>
Soumis le : mardi 10 octobre 2017 - 12:38:35
Dernière modification le : mardi 10 octobre 2017 - 13:45:46

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Marion Rouzaire, Aurélie Comptour, Corinne Belville, Damien Bouvier, Vincent Sapin, et al.. Cigarette smoke condensate affects the retinoid pathway in human amnion. Placenta, Elsevier, 2017, 58, pp.98 - 104. 〈10.1016/j.placenta.2017.08.076〉. 〈inserm-01614000〉

Partager

Métriques

Consultations de la notice

34