An alternative method for the determination of polysaccharide cleavage enzymes activities

Abstract : Most of usual methods used to detect and quantify polysaccharide cleavage enzymes (PCE) are not adapted to high throughput. In this work, enzymatic activities of three enzymes, namely an endocellulase (EC 3.2.1.4), an exocellulase (EC 3.2.1.91) and an alginate lyase (EC 4.2.2.3) were characterized by classical Michaelian model (Km, Vmax). Enzymatic activities were then determined by the use of the Enzyme Ring Test® (ERT®) method which allowed following the depolymerization of polysaccharides by PCE. Kinetic parameters were determined and compared to the ones obtained by classical methods. Besides, the ERT® technology clearly showed the possibility to discriminate endolytic and exolytic enzymatic mechanisms.
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Article dans une revue
Journal of Molecular Catalysis B: Enzymatic, Elsevier, 2015, 116, pp.166 - 172. 〈10.1016/j.molcatb.2015.03.019〉
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https://hal-clermont-univ.archives-ouvertes.fr/hal-01660669
Contributeur : Guillaume Pierre <>
Soumis le : lundi 11 décembre 2017 - 11:30:21
Dernière modification le : mardi 16 octobre 2018 - 10:48:01

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Véronique Tabone, Guillaume Pierre, Stéphanie Badel-Berchoux, Christine Gardarin, Thierry Bernardi, et al.. An alternative method for the determination of polysaccharide cleavage enzymes activities. Journal of Molecular Catalysis B: Enzymatic, Elsevier, 2015, 116, pp.166 - 172. 〈10.1016/j.molcatb.2015.03.019〉. 〈hal-01660669〉

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